Addgene

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pFTK043

Plasmid

#171315

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP fluorescent reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP1

Plasmid

#19044

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E26: attB2r-Halotag-attB3

Plasmid

#162915

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E14: attB4-EF1p>-attB5

Plasmid

#162975

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pre_IV_RFP_1317_v1

Plasmid

#126863

Purpose

1317 b scaffold based on pSB1A3 backbone with RFP gene as insert

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_RFP_1317_v1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proD

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSA107

Plasmid

#59785

Purpose

drives ZIF-1 protein in C. elegans ubiquitously

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

cdc-42 promoter (NEWENTRY Nematode)
zif-1 coding sequence (zif-1 )
unc-54 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Wnt5A STOP

Plasmid

#35874

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

adh1

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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Wnt11 STOP

Plasmid

#35885

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

WNT11 (WNT11 Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK019

Plasmid

#171291

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-10x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK017

Plasmid

#171289

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-5x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK038

Plasmid

#171310

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

hph, hygR, hygromycin selection marker

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK037

Plasmid

#171309

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

bleoR phleomycin, selection marker

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK039

Plasmid

#171311

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

pyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK084

Plasmid

#171356

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TtRNA[Met] A.niger Pol-III terminator

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK080

Plasmid

#171352

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Tcyc1 YJR048W terminator

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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