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1GFP/RNase H1 D210N
Plasmid#174448PurposeExpress GFP-tagged RNase H1 D210N in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationD210NPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ODD-Luciferase-pcDNA3
Plasmid#18965DepositorInsertODD-Firefly Luciferase (HIF1A Human)
UseTagsExpressionMammalianMutationHIF1a ODD sequence fused in frame to luciferase O…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 WT
Plasmid#121446Purposeexpression of huamn PD-L1 wildtype in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationwildtypePromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-BAZ2A
Plasmid#65373PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ2A (BAZ2A Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7f
Plasmid#104483PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7f
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mClover3-CD4-bla
Plasmid#179448PurposeDonor vector to knock in mClover3 N-terminal to human PER2 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_Syn_Xph20_eGFP_CCR5TC
Plasmid#187444PurposeEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseAAVTagseGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET iGluSnFR3 v857
Plasmid#175186PurposeFluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.DepositorInsertiGluSnFR3 v857
UseSynthetic BiologyTagsHis tag, T7 leader, Xpress epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mRuby2-CCR5TC
Plasmid#135531PurposeEncodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG-DATDCUE
Plasmid#185340PurposeAssessing domain requirement sof AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFLAG, 6xHisExpressionMammalianMutationdeletion of aa 90-337 corresponding to the acyl t…PromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG-DCUE
Plasmid#185339PurposeAssessing domain requirements of AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFLAG, 6xHisExpressionMammalianMutationdeletion of aa 293-337 corresponding to the CUE d…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mEos3.2-CCR5TC
Plasmid#135532PurposeEncodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmEos3.2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationsiRNA resistant and mutations A128V, T720A, A1347…PromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-BAZ1B
Plasmid#65372PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ1B (BAZ1B Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
tetO-AKT1
Plasmid#170682Purposedoxycycline-inducible overexpression of AKT1DepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin R21Q
Plasmid#51279Purposeexpresses human cofilin R21Q mutant fused to mRFP in mammalian cellsDepositorInsertcofilin 1 (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationR21Q, non-rod-forming mutation that can be used t…PromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Tol2 8xSTAR-mNeonGreen. PGK-puro
Plasmid#136255PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only
