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Plasmid#172491PurposemVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.DepositorInsertPdcd4 (Pdcd4 Mouse)
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterMLV LTRAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
Plasmid#158654Purposemaking transgenics expressing a QUAS driven YFP and NTR 2.0DepositorInsert5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7b
Plasmid#104484PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7b
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-BACE1-mBFP
Plasmid#196697PurposeExpresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertBeta-secretase 1 (BACE1 Human)
UseTagsHA and mBFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLX304-BCL-XL-Y195F
Plasmid#203573PurposeExpresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.DepositorInsertBCL2L1 (BCL2L1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationChanged Tyrosine 195 to Phenylalanine for partial…PromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1
Plasmid#120361PurposeProduce human recombinant TGFB1 in DE3 cellsDepositorInsertTransforming Growth Factor-β1 (TGFB1 Synthetic, Human)
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAi14-GFPNLS-MC
Plasmid#87114Purposeminicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITIDepositorInsertGFPNLSPpA
UseCRISPRTagsExpressionMutationPromoternEFAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX-N-CTCF-mAID-Halo-donor
Plasmid#139747PurposeMouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTagDepositorInsertCTCF CRISPR knock-in (Ctcf Mouse)
UseCRISPRTagsmAID-HaloTagExpressionMutationPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAi14-luc-MC
Plasmid#87113Purposeminicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITIDepositorInsertLuciferase-SV40pA
UseCRISPR and LuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#119187PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.DepositorInserthuman Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationtruncation after aa1945PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3
Plasmid#39484DepositorInsertARAP3 (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-BAZ1A
Plasmid#65371PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ1A (BAZ1A Human)
UseTagsEmGFP and V5ExpressionMammalianMutation796I compared to all reference sequencesPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro
Plasmid#132773PurposeExpression of human LMNA.DepositorInsertLamin A (LMNA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Twin-Strep-TEV-DGKζ
Plasmid#202531PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cellDepositorInsertDGKZ (DGKZ Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationPromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II tension sensor (F40-based)
Plasmid#118715PurposeThe F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-eGFP
Plasmid#62518PurposeExpresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.DepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dyn1(K44A)-mRFP
Plasmid#55795PurposeDominant negative Dynamin 1 mutant tagged with mRFPDepositorInsertDynamin-1(K44A)-mRFP (Dnm1 Discosoma sp., Rat)
UseTagsmRFPExpressionMammalianMutationDynamin-1(K44A)-mRFP was produced from Dynamin-1(…PromoterCMVAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only