Addgene

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pQF2wWB

Plasmid

#61313

Purpose

Enhancer trap version of QF2w, analogous to pGAWB

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF2w

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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Ntn1-AP-His

Plasmid

#71978

Purpose

Expresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn1 (Ntn1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI

Plasmid

#186559

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MND

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_SNAP_NR1

Plasmid

#170458

Purpose

Expresses SNAP tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-UNC5B-Fc(DAPA)-AviTag-6xHis

Plasmid

#156795

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

UNC5B (UNC5B Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

Plasmid

#117720

Purpose

Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

mAID-nanobody
IBB-mCherry

Use

Tags

importin-beta bining domain (IBB) and triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV and None, IRES-driven

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE8

Plasmid

#195569

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…

Tags

5' and 3' NLS

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBT373

Plasmid

#125614

Purpose

Expresses FERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

FERNY-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-142

Plasmid

#185931

Purpose

Himar transposon delivery plasmid that delivers a green fluorescent sGFP2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

sGFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG

Plasmid

#127301

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_WT

Plasmid

#81747

Purpose

Gateway Donor vector containing IRF4 ,part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/50R] (Recombinant Antibody)

Antibody

#183002-rAb

Purpose

Anti-CASK (Human) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Frog, Human, Mouse, Rat, and Zebrafish

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

May 31, 2022

Availability

Industry, Academic Institutions, and Nonprofits
2C-3XtbGFP-PEST

Plasmid

#69072

Purpose

Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

turboGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBAC-ECFP-15xQUAS_TATA-SV40

Plasmid

#104875

Purpose

piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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9X NFAT-apha-MHC-Luc

Plasmid

#51941

Purpose

9 copies of NFAT sites in minimal alphaMHC-pGL-3

Depositor

Jeffery Molkentin

Article

Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2

Inserts

9x NFAT binding site
alpha-MHC

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
Tornado-RNA sensor for MALAT-1

Plasmid

#185406

Purpose

circular RNA-scaffolded sensing modules for MALAT-1

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

U6

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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