Addgene

We narrowed to 19,026 results for: tes

Showing: 4881 - 4900 of 19026 results

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pET15b-pknG

Plasmid

#102814

Purpose

Expression of PknG in E.coli

Depositor

Jean Pieters

Article

Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

Insert

pknG

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-H123Y

Plasmid

#28299

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Phosphatase Inactive Mutant His123Tyr

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
RCAS Cyp26 (CC#62)

Plasmid

#15154

Depositor

Connie Cepko

Article

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

Cyp26 (CYP26A1 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
mCitrine-USP19 C506S

Plasmid

#78594

Purpose

Express mCitrine-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 C506S (USP19 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
pLK27

Plasmid

#37256

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

Scribble (SCRIB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1197-1630

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pSP64-AGS-mCherry

Plasmid

#135596

Purpose

Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Paxillin LD

Plasmid

#186150

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D5A; D146A; D232A; D267A; D301A

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pECFP-Sec23A

Plasmid

#66610

Purpose

CFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pSLIK 3XFLAG-EGFP-NLS hygro

Plasmid

#87781

Purpose

Inducible expression of EGFP with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL

Plasmid

#23103

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pGSTag Bim EL (Bcl2l11 Mouse)

Use

Tags

GSTag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pPNT6-eGFP

Plasmid

#71303

Purpose

This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression

Depositor

Gian Luca Forni, Marco Musso

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Mis12 INCENP Δcen mCherry

Plasmid

#45228

Depositor

Michael Lampson, Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 INCENP Δcen mCherry

Use

Tags

VSVG

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pBlueRIP.OVA

Plasmid

#69597

Purpose

contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)

Depositor

Francis R. Carbone

Article

Blanas et al Science. 1996 Dec 6;274(5293):1707-9.

Insert

OVA (OVAL Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

rat insulin promoter (RIP)

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rap1b S179AS180A

Plasmid

#118327

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b S179AS180A (RAP1B Bovine)

Use

Tags

GFP

Expression

Mammalian

Mutation

S179AS180A

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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delta Zfp521

Plasmid

#104190

Purpose

Expression of delta Zfp521 in mammalian cells

Depositor

Kathryn Hentges

Article

AlDallal et al Leuk Res. 2016 Jul;46:10-7. doi: 10.1016

Insert

Zfp521 (Zfp521 Mouse)

Use

Tags

Expression

Mammalian

Mutation

deletion of final 6 Zn fingers

Promoter

CMV

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDest472 SHP-1 S591D

Plasmid

#21806

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 591 to Aspartic Acid

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only