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Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab9 DN
Plasmid#12664DepositorInsertRAB9 (Homo sapiens) (RAB9A Human)
UseTagsGFP and HAExpressionMammalianMutationchanged serine 21 to asparaginePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
UseTagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 E76A
Plasmid#8331DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationE76A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 ∆24-mCherry
Plasmid#80271Purposemammalian expression plasmid for Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT PUR mGSK3AWT
Plasmid#35159DepositorInsertGSK-3α (Gsk3a Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pR008_APAD
Plasmid#204818PurposeExpression Apobec1-ADAR2dd fusion protein as PIE-RBP controlDepositorInsertrat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
UseTagsHA and Flag and huADAR2ddExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A
Plasmid#102585PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-Electra2
Plasmid#179477PurposeER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450DepositorInsertCytERM-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-FLAG-NEK9
Plasmid#168269PurposeExpresses NEK9 in mammalian cellsDepositorInsertNEK9 (NEK9 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#50942PurposeBicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-Electra1-P2A-mScarlet
Plasmid#196113Purposezebrafish expression of nbt-Electra1-P2A-mScarletDepositorInsertnbt-Electra1-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_3XNLS-MicroID2-FLAG
Plasmid#188523PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cellsDepositorInsert3XNLS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 K232R
Plasmid#80878Purposemammalian expression of ALK5 K232RDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationK232R (kinase dead)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA
Plasmid#158972PurposeVector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-Pepper-RhoBAST
Plasmid#196343PurposeOverexpression of circular Pepper-RhoBASTDepositorInsertTornado-Pepper-RhoBAST
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-WDR5
Plasmid#200880PurposeMammalian expression of human WDR5 with N-terminal HaloTag fusionDepositorInsertHaloTag-WDR5 (WDR5 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FKBP-FKBP-CFP (LF2C)
Plasmid#20149DepositorInsertLyn-FKBP-FKBP-CFP (FKBP1A Human)
UseTagsExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Alu-Lin28
Plasmid#92346PurposePlasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.DepositorInsertLin28 gene derived Alu element containing 3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only