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Plasmid#219742PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK189
Plasmid#219743PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiensDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK4169
Plasmid#219744PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastorisDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyTagsExpressionMutationD37E, V181I, S323M, M385KPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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AAV2_CAG_oROS-HT(C199S)_WPRE
Plasmid#216415PurposeEncodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectorsDepositorInsertoROS-HT(C199S)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-gp78 RING
Plasmid#185353PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorInsertgp78 cytoplasmic domain (AMFR Human)
UseTagsGSTExpressionBacterialMutationStop codon after aa 393PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD
Plasmid#196878PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-AERRIE
Plasmid#204023PurposeLentiviral vector expressing human long non-coding RNA AERRIE (linc01013)DepositorInsertlong non-coding RNA AERRIE (LINC01013 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-AKT1
Plasmid#170682Purposedoxycycline-inducible overexpression of AKT1DepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-HAND1
Plasmid#170689Purposedoxycycline-inducible overexpression of HAND1DepositorInsertHAND1 (HAND1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-gp78 429-611
Plasmid#185354PurposeGST fusion protein for quantification of gp78 levelsDepositorInsertgp78 (AMFR Human)
UseTagsGSTExpressionBacterialMutationaa 429-611 onlyPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 292-410 5E
Plasmid#185332PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 only, with mutations R382E/Q383E/K390E…PromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 292-410 A397D
Plasmid#185333PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 only, with mutation A379DPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD
Plasmid#196881PurposeNeuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G2BRgp78
Plasmid#185344PurposeExpression of soluble G2BR, amino acids 575-600 of human gp78DepositorInsertgp78 aa 574-600 (G2BR) (AMFR Human)
UseTagsEGFPExpressionMammalianMutationaa 575-600 onlyPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorInsertAUP1 aa 379-410, Ube2G2 (AUP1 Human, Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG-DCUE
Plasmid#185339PurposeAssessing domain requirements of AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFLAG, 6xHisExpressionMammalianMutationdeletion of aa 293-337 corresponding to the CUE d…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA10-mVenus
Plasmid#185648PurposeNDUFA10 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA10 (NDUFA10 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA5-mVenus
Plasmid#185646PurposeNDUFA5 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA5 (NDUFA5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFB6-mVenus
Plasmid#185652PurposeNDUFB6 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFB6 (NDUFB6 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only