Addgene

We narrowed to 14,108 results for: LEA

Showing: 4861 - 4880 of 14108 results

Results per page:

pGEX-PP-NEDD4L-WW2(385-418)

Plasmid

#166702

Purpose

expresses NEDD4L WW2 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW2 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-NEDD4L-WW3(497-530)

Plasmid

#166703

Purpose

expresses NEDD4L WW3 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW3 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-NEDD4L-WW4(548-581)

Plasmid

#166704

Purpose

expresses NEDD4L WW4 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW4 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Availability

Academic Institutions and Nonprofits only

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pCA528-PPxY1-GS-WW3e

Plasmid

#166705

Purpose

expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

AMOT PPxY1 fused to NEDD4L WW3

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW1

Plasmid

#166707

Purpose

expresses NEDD4L with inactivating mutation in first WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W221A

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 221 to alanine

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW2

Plasmid

#166708

Purpose

expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W453A

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 453 to alanine

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW3

Plasmid

#166709

Purpose

expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W525A

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 525 to alanine

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW4

Plasmid

#166710

Purpose

expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W576A

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 576 to alanine

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY1

Plasmid

#166711

Purpose

expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 L129A P130A

Use

Tags

2xHA

Expression

Mammalian

Mutation

changed leucine 129 to alanine, proline 130 to al…

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Article

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCAG-spCas9-N57

Plasmid

#169920

Purpose

Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

SpCas9-N57

Use

CRISPR

Tags

Expression

Mutation

N57 is the PAI domain of SB100X

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pH-mono-MAD7

Plasmid

#170134

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al J Genet Genomics. 2021 May 3. pii: S1673

Insert

MAD7(ErCas12a)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

maize Ubiquitin-1, switchgrass Ubiquitin-1

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Use

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Promoter

Availability

Academic Institutions and Nonprofits only

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His-SUMO-Rad23A_pET11a

Plasmid

#169124

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hRad23A (RAD23A Human)

Use

Tags

Expression

Bacterial

Mutation

wt

Promoter

Availability

Academic Institutions and Nonprofits only

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pSin-GFP-Fg

Plasmid

#174307

Purpose

Lentiviral expression of GFP

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GFP (eGFP Aequorea victoria)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pBS-tRNA-Gly

Plasmid

#174317

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-gly

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBS-tRNA-Ser

Plasmid

#174318

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-ser

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-TM

Plasmid

#176886

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

Ig kappa-FRISZ-PDGFR TM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pYPQ220-Ultra

Plasmid

#176889

Purpose

Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

AsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pYPQ230-E795L

Plasmid

#176890

Purpose

Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

Depositor

Yiping Qi

Article

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

LbCas12a-E795L

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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