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Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralTagsExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 GFP
Plasmid#160680Purposevector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN382 (FuGW-S(HIF1A)p-mKate2 )
Plasmid#105180Purposesynthetic promoter HIF1A drives mKate2 expressionDepositorInsertS(HIF1A)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YC
Plasmid#74724PurposeExpress C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-Halo-iLID
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR651
Plasmid#65213PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-1 cap
UseTagsExpressionInsectMutationPromoterp10 and polyhedrinAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[N150TAA]
Plasmid#164581Purposesuperfolder GFP with N150TAA mutationDepositorInsertsfGFP-150TAA
UseTags6xHisExpressionBacterialMutationN150TAA MutationPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MEF2C-HA
Plasmid#32515DepositorInsertMef2c (Mef2c Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
iLID-mCl3-MAP7N
Plasmid#190167PurposeExpresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertiLID-mClover3-MAP7N
UseTagsiLID-mClover3ExpressionMammalianMutationaa 1-309 from human MAP7 delta175-213PromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mKdm4d-polyA
Plasmid#61553PurposeFor mRNA synthesis or expression in mammalian cellsDepositorInsertKdm4d (Kdm4d Mouse)
UseTagsflagExpressionMammalianMutationPromoterCMV and T7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb80
Plasmid#205519PurposeNuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertnuc-Nb80
UseTagsNuclear localization signal (NLS) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-Kaede
Plasmid#132972Purposefull-length of photoconvertible kaede with stop codon in pmE Gateway vectorDepositorInsertKaede
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-SDHB-Flag
Plasmid#50055PurposeExpression of human SDHB in mammalian cellsDepositorInsertSDHB (SDHB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only