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Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEn-C1.1
Plasmid#61479PurposeEncodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPCDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluh
Plasmid#119830PurposeProduction of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluh
UseTagsGSTExpressionBacterialMutationE25APromoterT7Available sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
UseTagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialMutationPromotertacAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Bla(HA–RILP-FLAG)
Plasmid#102424PurposeDual tagged RILP for expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterTet-ResponsiveAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36β
Plasmid#214344PurposeProtein overexpression in bacteriaDepositorInsertIL36B (IL36B Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-1333-end
Plasmid#46070DepositorInsertMap7-1333-end (Map7 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC102
Plasmid#118883Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorInsertALFY (WDFY3 Human)
UseTags6xHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Nd1
Plasmid#191523PurposeExpression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vectorDepositorInsertv8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52545PurposeTargeting vector for homologous recombination in mouse ES cells; Hipp11 locusDepositorInsertsPhiC31o attP (full length)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAGFP
Plasmid#58255PurposeU6 promoter-driven single guide RNA complementary to eGFPDepositorInsertU6.gRNAGFP cassette
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_PPVs
Plasmid#119208PurposeCircularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with PPVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMutationPromoterCMVAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInsert-PhoCl-RER
Plasmid#219676PurposeCaging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2DepositorInsertCD4mScarlet-PhoCl-RER
UseTagsmScarlet and PhoClExpressionMammalianMutationPromoterTREAvailable sinceJune 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet-1_VNp15-DARPinOFF7
Plasmid#182399PurposeBacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusionDepositorInsertVNp15-DARPinOFF7
UseTagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
UseTagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only