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pAAV9-hSyn-iCytSnFR_ER

Plasmid

#177752

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCytSnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_ER

Plasmid

#177756

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_ER

Plasmid

#177754

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDL128

Plasmid

#43912

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pDL127

Plasmid

#43911

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCY 3120-04

Plasmid

#36238

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-13Myc-TRP1

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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VTA

Plasmid

#27805

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Venus Traf Amber

Expression

Mammalian

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6.3-Eevee-iAkt-FR

Plasmid

#252349

Purpose

Lentiviral transfer vector with Eevee-iAkt-FR.

Depositor

James Conway

Article

Tran et al Small Methods. 2026 Jul 12:e70828. doi:

Insert

Eevee-iAkt-FR

Use

Lentiviral

Promoter

CMV

Available Since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-miRFP670nano3

Plasmid

#252339

Purpose

Transient expression of miRFP670nano3.

Depositor

James Conway

Article

Tran et al Small Methods. 2026 Jul 12:e70828. doi:

Insert

miRFP670nano3

Tags

FLAG and V5; 6xHis

Expression

Mammalian

Promoter

EF1a

Available Since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rACh1l-IRES-EGFP-CAAX

Plasmid

#234418

Purpose

Expresses the red ACh sensor GRAB rACh1l and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Xie et al Nat Neurosci. 2026 Jun 16. doi: 10.1038/

Insert

GRAB-rACh1l

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pYS108

Plasmid

#255530

Purpose

Bacterial expression of W163(5HTP) variant of human γS-crystallin

Depositor

Rachel Martin

Article

Seo et al Biophys Rep (N Y). 2026 Mar 11;6(1):1002

Insert

W163(5HTP) variant of human γS-crystallin (CRYGS Human)

Expression

Bacterial

Mutation

Tryptophan 163 changed to 5-hydroxytryptophan

Promoter

T7

Available Since

June 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-VSG-228

Plasmid

#245117

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-VSG-228-telo

Plasmid

#245104

Purpose

Inserts VSG-228 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLAREmc1

Plasmid

#254652

Purpose

AAV vector for expressing FLAREmc1 sensor under synapsin promotor.

Depositor

Stephane Laporte

Article

Namkung et al Mol Metab. 2025 Nov;101:102254. doi: 10.

Insert

FLAREmc1 melanocortin sensor

Use

AAV

Promoter

human synapsin

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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FSW TMEM192-3xHA

Plasmid

#246729

Purpose

Neuron-specific expression of human TMEM192 with a tag of 3x HA on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

TMEM192 (transmembrane protein 192) (TMEM192 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

Synapsin

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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