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Plasmid#160958PurposeMki67 shRNA in pMKO.1 retroviral vectorDepositorInsertMki67 (Mki67 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE
Plasmid#129704PurposeExpress HA-KASH for nuclei labeling and purificationDepositorInsertHA-VHH-KASH
UseAAV and Cre/LoxTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCer-Vamp2(qv/fw)
Plasmid#53238PurposeMutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationMutated Q76 to V; F77 to W.PromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
3535 pET15b Bid p22
Plasmid#8784DepositorInsertBid p22 (Bid Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorInsertECFP-FHA1-JNKsubDD-Citrine-NES
UseTags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKC-delta-IRES-hrGFP
Plasmid#73414PurposeMammalian expression of mouse PKC-delta and hrGFPDepositorInsertPKC-delta (Prkcd Mouse)
UseTags3xFLAG and IRES-hrGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-Flag-ZO-2
Plasmid#27420DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1p shDna2
Plasmid#31951DepositorInsertshRNA against hDna2 (DNA2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 Srap1
Plasmid#89691PurposeExpresses murine Srap1 in bacteriaDepositorInsertMurine SRAP domain ortholog Srap1 (Hmces Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
IBMc071
Plasmid#161943PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmidDepositorInsertpSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
R26-EGFP MMEJ donor vector
Plasmid#137926PurposeR0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based methodDepositorInsertSA-EGFP-bpA with micro-homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-deltaC
Plasmid#21123DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutation5 Carboxyterminal amino acids -FLTWL are deletedPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pY006 (pcDNA3.1-hBpCpf1)
Plasmid#69978PurposeExpresses humanized BpCpf1DepositorInserthBpCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
3495 pcDNA3 Bid p15
Plasmid#8780DepositorInsertBid p15 (Bid Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pY013 (pcDNA3.1-hEeCpf1)
Plasmid#69985PurposeExpresses humanized EeCpf1DepositorInserthEeCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cas9-VPR
Plasmid#163971PurposeTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeastDepositorInsertCas9-VPR
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1
Plasmid#166711Purposeexpresses AMOT with inactivating mutation in first PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 L129A P130A
UseTags2xHAExpressionMammalianMutationchanged leucine 129 to alanine, proline 130 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAYC
Plasmid#62657PurposeMammalian expression of iCre recombinase from the broadly active CAG promoter/enhancerDepositorInsertiCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only