Addgene

We narrowed to 27,949 results for: eng

Showing: 4781 - 4800 of 27949 results

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AAV8-cPE-N

Plasmid

#183538

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

N-terminal fragment of SpCas9 nickase (H840A)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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CCR5_gRNA_pX459

Plasmid

#192268

Purpose

gRNA expression for CRISPR Genome Editing of CCR5

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

CCR5 guide RNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-6A-HA

Plasmid

#128103

Purpose

Express HA tagged Nxph4-6A

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRKB134_GI-DUX4-intron2(-)

Plasmid

#83968

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(-) (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

mouseCMV

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pRKB133_GI-DUX4-intron2(+)

Plasmid

#83967

Purpose

to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

Depositor

Robert Bradley

Article

Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

Insert

DUX4-3'UTR-intron2(+) (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

mouseCMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
pSin-NE1m

Plasmid

#172883

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

Depositor

J. Julius Zhu

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Insert

NE SENSOR

Use

Tags

Expression

Mammalian

Mutation

Promoter

sp6

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EE-mut SV40 luc

Plasmid

#8460

Depositor

Bob Weinberg

Article

Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

Insert

human cyclin E promoter fragment, mutant (CCNE1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

E2F sites I, II, and III mutated.

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
p3×FLAG-CMV-14-LanCL2

Plasmid

#73391

Purpose

Expresses FLAG tagged LanCL2

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

LanCL2 (LANCL2 Human)

Use

Tags

3x FLAG tagged

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Wnt3a (CT#169)

Plasmid

#13976

Depositor

Cliff Tabin

Article

Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

Insert

Wnt3a (Wnt3a Mouse)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
dpYPQ230-D156R-CBE2

Plasmid

#195368

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…

Tags

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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MCV-Rep-

Plasmid

#32058

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-Rep- genome

Use

Tags

Expression

Mutation

Single cytosine to adenine (C/A) mutation at 2,07…

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pJKW1813

Plasmid

#174003

Purpose

Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP90G4
ATR1 (ATR1 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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FUP95PDZGW (C5)

Plasmid

#74021

Purpose

lentiviral expression of Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

PDZ

Promoter

hUbC

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
pVMG0146_Cq-U6-1_2xBbsI-gRNA

Plasmid

#169238

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

Cq-U6-1_2xBbsI-gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039653

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0149_Cq-U6-6_2xBbsI-gRNA

Plasmid

#169323

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0149_Cq-U6-6_2xBbsI-gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039596

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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