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Plasmid#198759Purposeconditional knockdown of FDPSDepositorInsertshFDPS #1 (FDPS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-4x mut_R
Plasmid#147371PurposeMammalian Expression of HsNot1iso1-4xmutDepositorInsertHsNot1iso1-4xmut (CNOT1 Human)
UseTagsExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-K1208AH1212AK1218A_R
Plasmid#147372PurposeMammalian Expression of HsNot1iso1-K1208AH1212AK1218ADepositorInsertHsNot1iso1-K1208AH1212AK1218A (CNOT1 Human)
UseTagsExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-K1208EH1212TK1218E_R
Plasmid#147373PurposeMammalian Expression of HsNot1iso1-K1208EH1212TK1218EDepositorInsertHsNot1iso1-K1208EH1212TK1218E (CNOT1 Human)
UseTagsExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R
Plasmid#147374PurposeMammalian Expression of HsNot1iso1-P1209YP1257YDepositorInsertHsNot1iso1-P1209YP1257Y (CNOT1 Human)
UseTagsExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-P1257Y_R
Plasmid#147375PurposeMammalian Expression of HsNot1iso1-P1257YDepositorInsertHsNot1iso1-P1257Y (CNOT1 Human)
UseTagsExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsDCP1a-Y36A_P
Plasmid#147218PurposeMammalian Expression of HsDCP1a-Y36ADepositorInsertHsDCP1a-Y36A (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and G1716T compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-Y36A_P
Plasmid#147221PurposeMammalian Expression of HsDCP1a-Y36ADepositorInsertHsDCP1a-Y36A (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_1-1089_K
Plasmid#146804PurposeMammalian Expression of HsNot1iso1_1-1089DepositorInsertHsNot1iso1_1-1089 (CNOT1 Human)
UseTagsExpressionMammalianMutationDeletion of 5 AA compared to the sequence given b…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_1085-1605_K
Plasmid#146805PurposeMammalian Expression of HsNot1iso1_1085-1605DepositorInsertHsNot1iso1_1085-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1089_K
Plasmid#146806PurposeMammalian Expression of HsNot1iso1_1-1089DepositorInsertHsNot1iso1_1-1089 (CNOT1 Human)
UseTagsExpressionMammalianMutationDeletion of 5 AA compared to the sequence given b…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1085-1605_K
Plasmid#146807PurposeMammalian Expression of HsNot1iso1_1085-1605DepositorInsertHsNot1iso1_1085-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-Halo-Dync1h1motor
Plasmid#191333PurposeExpresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-Dync1h1motor
Plasmid#191332PurposeExpresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-Dync1h1motor (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ZPBP-COMP5AP-AviTag-9xHis
Plasmid#157311PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertZPBP (ZPBP Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-Y373C
Plasmid#191754PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-Y373C mutation (associated with TD1) (target mutation: c.1118A>G ; silent mutation added on purpose: c.1110C>A)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1118A>G, p.Y373C + c.1110C>A (silent muta…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-1
Plasmid#190095Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-1 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 6704-6709 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-2
Plasmid#190096Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-2 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 7122-7129 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-PA-GFP-RhoAQ63L
Plasmid#187282PurposeExpress pmRFP-PAGFP-RhoA Q63LDepositorInsertRhoA (RHOA Human)
UseTagsmRFP-PAGFPExpressionMammalianMutationRhoA Q63LPromoterCMV, T7Available sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-JD
Plasmid#184247PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)DepositorInsertAtaxin-3 Josephin domain (ATXN3 Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon V183XPromoterT7 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only