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Plasmid#199477PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-g418-His:mScarletI:His
Plasmid#201098PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-His:mScarletI:His
Plasmid#201097PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-g418-His:mNG:His
Plasmid#201095PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-tagRFPt
Plasmid#201085PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserttagRFPt
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-tagRFPt
Plasmid#201084PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserttagRFPt
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SV40-iBlue-pA
Plasmid#200234PurposeBackbone for cloning iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-eYFP
Plasmid#190219PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserteYFP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mGL
Plasmid#190221PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmGreenLantern
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6.1-phleo-Ty:mNG:Ty
Plasmid#181936PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10V5
Plasmid#181938PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xV5
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-HaloTag
Plasmid#181953PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertHaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9_P2A_POLB
Plasmid#190145PurposeExpresses SpCas9 and POLBDepositorInsertSpCas9_P2A_POLB
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNA
Plasmid#194724Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target tdTomatoDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-tdTomato-sg
Plasmid#194722PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomatoDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-GFP-sg
Plasmid#194717PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFPDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP1411
Plasmid#167638PurposeExpress FepA I14C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA I14C
UseTagsExpressionBacterialMutationfepA with I14C mutation (Mutation is I36C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1383
Plasmid#167639PurposeExpress FepA V15C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V15C
UseTagsExpressionBacterialMutationfepA with V15C mutation (Mutation is V37C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1384
Plasmid#167641PurposeExpress FepA V16C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V16C
UseTagsExpressionBacterialMutationfepA with V16C mutation (Mutation is V38C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1416
Plasmid#167643PurposeExpress FepA T17C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T17C
UseTagsExpressionBacterialMutationfepA with T17C mutation (Mutation is T39C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only