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Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-pMagHigh-FlpC
Plasmid#180586PurposePA-FlpDepositorInsertSplit FlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh
Plasmid#180585PurposePA-FlpDepositorInsertSplit FlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
RFP-PASS
Plasmid#193971PurposeMonitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)DepositorInsertPA biosenor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GTB
Plasmid#26197DepositorInsertEnhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationTVA, rabies glycoprotein are mammalian codon opti…PromoterAvailable sinceNov. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
Plasmid#198753PurposeCre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activityDepositorInsertEF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
UseLentiviralTagsExpressionBacterial and MammalianMutationSee depositor comments belowPromoterEF1aAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pbG-APRT-J-u32uni-PdTKmCh
Plasmid#107279PurposeAPRT c.407T>C (APRT*J) donor vector with unilateral microhomologyDepositorInsertsUseTagsExpressionMutationc.402A>T, c.407T>CPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ai14-luc
Plasmid#87118PurposeAAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITIDepositorInsertU6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
UseAAV, CRISPR, and LuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rMERTK-HITI
Plasmid#87119PurposeGene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITIDepositorInsertU6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only