Addgene

We narrowed to 14,672 results for: LEA

Showing: 461 - 480 of 14672 results

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pLentiPGK Hygro DEST ERKKTRmCerulean3

Plasmid

#90229

Purpose

Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2-Venus

Plasmid

#138196

Purpose

FRET-based biosensor for monitoring Epac2 activation.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (Cerulean-Venus)

Plasmid

#18679

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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raACR-mCerulean3-pAAV2-hSyn

Plasmid

#204966

Purpose

Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

raACR

Use

AAV

Tags

mCerulean3

Expression

Mutation

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344A-mCerulean

Plasmid

#69580

Purpose

Expresses p53-L344A mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (EGFP-mRFP)

Plasmid

#18682

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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Cerulean-tagged Apollo-NADP+

Plasmid

#71800

Purpose

Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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V5-BS2-SENP2 (Nuclear Pore)

Plasmid

#220345

Purpose

Mammalian expression vector BS2 in nuclear pore

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Use

Tags

SENP2 (nuclear pore localized protein) and V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAF018 p3E-2A-mCerulean-CAAX

Plasmid

#195975

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Article

Kirchgeorg et al Dev Dyn. 2018 Oct;247(10):1146-1159. doi

Insert

2A-mCerulean-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pOpen-Exonuclease III E. coli

Plasmid

#165571

Purpose

Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Exonuclease III E. coli

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-BiPOLES-mCerulean

Plasmid

#154950

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn-Flex RG-cerulean

Plasmid

#98221

Purpose

Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus

Depositor

Troy Margrie

Insert

Cerulean-E2A-RG

Use

AAV

Tags

cerulean

Expression

Mutation

Promoter

human Synapsin

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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769 pME mCerulean-CAAX

Plasmid

#195957

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mCerulean-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish ubiquitin promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Luciferase-IRES-mCerulean

Plasmid

#75022

Purpose

Encodes the expression of Luciferase and mCerulean

Depositor

Saradhi Mallampati

Insert

Luciferase

Use

Luciferase and Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only