Addgene

We narrowed to 638 results for: Bacillus

Showing: 461 - 480 of 638 results

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YE1-BE4max-NG

Plasmid

#138159

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLH01

Plasmid

#117261

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-Lp_0640-42, sapK-sappR

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLH02

Plasmid

#117262

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-LVISKB_1732-30 , sapK-sappR

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pG1AK-PheB

Plasmid

#71738

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

PheB

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Use

Tags

Expression

Bacterial

Mutation

Promoter

sppA

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-G9QLF2

Plasmid

#103111

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

G9QLF2(Cas9 coding gene from Bacillus smithii 7_3_47FAA)

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Use

Tags

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

PEP-carboxykinase promoter

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Article

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Use

Tags

Expression

Bacterial

Mutation

Promoter

sppA

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLPR3

Plasmid

#209986

Purpose

Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (ermBL)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPS2

Plasmid

#209972

Purpose

Contains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

RBS (Lp)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPR1

Plasmid

#209979

Purpose

Contains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (ermBL)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPCK

Plasmid

#133128

Purpose

a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

PEP-carboxykinase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

PEP-carboxykinase promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMDH

Plasmid

#133129

Purpose

A recombinant plasmid expressing mdh (malate dehydrogenase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

malate dehydrogenase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

malate dehydrogenase promoter

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkaš et al Engineering Microbiology, 2022

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA251_ Ptrc.x.tetO2::D-HicDH

Plasmid

#185456

Purpose

CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

Depositor

Wolfgang Kroutil

Article

Jurkaš et al Engineering Microbiology, 2022

Insert

D-HicDH from Lactobacillus paracasei DSM 20008

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc.x.tetO2

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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