Addgene

We narrowed to 23,060 results for: GFP

Showing: 4741 - 4760 of 23060 results

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pORANGE GFP-Nlgn3 KI

Plasmid

#131501

Purpose

Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Use

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pLenti_CMV-EGFP-2A-mNeonGreen

Plasmid

#171599

Purpose

Green lentiviral vector plasmid for pseudovirus experiments

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

EGFP
mNeonGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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PL-SIN-PGK-EGFP

Plasmid

#21316

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-ACTB-mEGFP

Plasmid

#115917

Purpose

donor to insert EGFP at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-mEGFP

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CaMKIIbeta(A303R)

Plasmid

#21224

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(A303R) (Camk2b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(A303R) (Lab plasmid CF 0002)

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV::hTfR-GFP

Plasmid

#199714

Purpose

Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

hTfR-GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
CMV:AsCpf1-2A-GFP-U6-AAVS1-sg

Plasmid

#194716

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD053 GFP KIKO

Plasmid

#192156

Purpose

GFP KIKO

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

GFP

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-eGFP

Plasmid

#179781

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

eGFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLEGFP-K685R-STAT3

Plasmid

#71449

Purpose

Retroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

K685R

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
NLS-LucDM-GFP

Plasmid

#127796

Purpose

The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

destabilized mutant (DM) Luciferase

Use

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

R188Q, R261Q in Firefly Luciferase insert

Promoter

CMV

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-uTEV1-NES

Plasmid

#171006

Purpose

HiLITR protease with C-terminal NNES (Cytoplasmic)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-NES

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-cDAXX-0SB

Plasmid

#169728

Purpose

Mammalian expression construct of GFP tagged cDAXX-0SB

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX-0SB (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Comprises residues 495-741 and has the major SB m…

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-LucWT-GFP

Plasmid

#127709

Purpose

The expressed ORF encodes nucleus location signal with GFP and WT Luciferase.

Depositor

Xiaolu Yang

Article

Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

Insert

Luciferase

Use

Tags

EGFP and FLAG-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-TOMM20-N-10

Plasmid

#57158

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits