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Plasmid#167355PurposeIntegrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1tDepositorInsert4xUAS-SSA1p-365:yEGFP3-CYC1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS306:VN
Plasmid#37555DepositorInsertVenus N-term (aa 1-272)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorArticleInsertyeGFP
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterpGRR-r11-r10Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Hp53
Plasmid#72242PurposeContains the CORE cassette with hyg and human p53 gene markersDepositorInsertsUseTagsExpressionBacterialMutationV122A mutationPromoterAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB5191
Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_TwinStrep-GFP11
Plasmid#153514PurposeTwinStrep-GFP11 C-terminal Tag Donor PlasmidDepositorInsertTwinStrep-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PSUC2-yEGFPCLN2PEST-pRS406
Plasmid#64405PurposeSUC2 promoter driven GFP expressionDepositorInsertSUC2 promoter (SUC2 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterSUC2Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-2xOsh2 PH domain [short]
Plasmid#58829PurposeExpresses GFP-Tagged Osh2 PH domain in yeastDepositorInsert2xOsh2 PH domain [short] (OSH2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterADH1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP363
Plasmid#78984Purpose255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.DepositorInsertsrHGH
IFNa2b
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDYSCKO
Plasmid#120263PurposeEmpty backbone for cloning any gRNA to be used with the canavanine co-selection systemDepositorInsertDYSCKO cassette
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSNR52Available sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-trAtCCD8(pYL736)
Plasmid#178287PurposeExpresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F53
Plasmid#23077DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Arginine, H3 K9RPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F30
Plasmid#23066DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed lysine 9 to Glutamine H3 K9QPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAH1
Plasmid#41825PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F31
Plasmid#23067DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed lysine 14 to Glutamine K14QPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only