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Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Neo_CAG-Cas9
Plasmid#86698PurposeCas9 expression vector for targeting to the AAVS1 locusDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterpCagAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BeGFP
Plasmid#21210DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-eGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-mRFP
Plasmid#40996DepositorInsertmRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9663 - ACTB GFP-P2A CLIP Donor
Plasmid#202688PurposeCLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.DepositorInsertCopGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo
Plasmid#204669PurposeMammalian expression of membrane-bound Pink Flamindo for cAMP imagingDepositorArticleInsertMyr-Pink Flamindo
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CMV sTurboID (C)-Arrestin-GFP
Plasmid#153010PurposeExpresses split-TurboID C-terminal fragment fused to arrestinDepositorInsertsTurboID (C)(G74)-Arrestin
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV1 trial size)
Viral Prep#50465-AAV1.TPurposeReady-to-use AAV1 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-DUB3
Plasmid#22593DepositorInsertDUB3 (USP17L9P Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMarC9-R6k
Plasmid#89477Purposetransposon plasmid, compatible with Tn-SeqDepositorInsertpMarC9-R6k
UseTagsExpressionMutationPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-PIP-FUCCI
Plasmid#138714PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sRGECO (AAV8)
Viral Prep#137125-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-sRGECO (#137125). In addition to the viral particles, you will also receive purified pAAV-Ef1a-sRGECO plasmid DNA. EF1a-driven expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP_CD47_LU
Plasmid#65474PurposeGFP tagged CD47 with its own long 3'UTRDepositorInsertsSignal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A315T
Plasmid#133755PurposeLentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged alanine 315 to threoninePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse sgRNA library Brie in lentiCRISPRv2
Pooled Library#73632PurposeMouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1500
Plasmid#143960PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBL2 (RBL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only