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Showing: 4661 - 4680 of 13594 results

  1. GB1-PRD(703-868, S712C, P801G, C813S)-Strep

    Plasmid
    #164445
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Serine 712 to Cysteine, Proline 801 to Glycine, C…
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164445-plasmid-map-sequence-id-324032

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  2. GB1-PRD(703-868, A756C, P801G, C813S)-Strep

    Plasmid
    #164446
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Alanine 756 to Cysteine, Proline 801 to Glycine, …
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164446-plasmid-map-sequence-id-324033

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  3. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

    Plasmid
    #164447
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Cysteine 813 to Serine, S…
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164447-plasmid-map-sequence-id-324034

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  4. FN(9-10, RGE)-I27-CC

    Plasmid
    #85396
    Purpose
    MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    GRGDS motif mutated to GRGES
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85396-plasmid-map-sequence-id-172700

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  5. KS6A1_HUMAN_D1

    Plasmid
    #79720
    Purpose
    This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Nicholas Levinson, Markus Seeliger, John Chodera
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KS6A1 (RPS6KA1 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. KC1G3_HUMAN_D0

    Plasmid
    #79685
    Purpose
    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Nicholas Levinson, Markus Seeliger, John Chodera
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KC1G3 (CSNK1G3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Inserts
    Gaussia luciferase, slow burn
    Volvox Channelrhodopsin 1
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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  20. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162681-plasmid-map-sequence-id-321444

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Showing: 4661 - 4680 of 13594 results