His-GIV-CTs
(Plasmid #69864)

• Purpose: Express His tagged GIV-CTs (1660–1870).
• Depositing Lab: Pradipta Ghosh
• Publication: Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86. doi: 10.1091/mbc.E10-08-0738. Epub 2011 Jan 5.

Backbone

• Vector backbone: pET-28b
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 630
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GIV/Girdin
• Alt name: His-GIV-CTs
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Mutation: C terminal amino acids S1660-S1870 of human GIV.
• Entrez Gene: CCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry