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Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-FLAG-APEX2-NES-TRP1
Plasmid#229228PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-min5UTR-hRluc-A60
Plasmid#182638PurposeTranscription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase,
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199450PurposeCRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199453PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)
Plasmid#199451PurposeCRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR regionDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)
Plasmid#199452PurposeCRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY237
Plasmid#184150PurposeReprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)DepositorInserts-tracrRNA-R1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY251
Plasmid#184153PurposeReprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)DepositorInserts-tracrRNA-Ar2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY283
Plasmid#184156PurposeReprogrammed short tracrRNA for hijacking mRNA of zntADepositorInserts-tracrRNA-zntA-1034
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISH-OMACS
Plasmid#136765PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Acsm4DepositorInsertAcsm4 (Acsm4 Mouse)
UseIn situ probeTagsExpressionMutationISH Probe insert, A102T- please see depositor com…PromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA20
Plasmid#158498PurposeIn planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationL17E, D489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HoxC-downstream-5xTetO Donor
Plasmid#131341PurposeDonor template to insert 5xTet operator sequence into downstream of HoxC cluster.DepositorInsertHoxC-downstream-5xTetO Donor
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only