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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTU1-B-lacZ

Plasmid

#72936

Purpose

Level 1 Destination vector - Position B

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - B site

Use

Synthetic Biology

Tags

Expression

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pTU3-A-RFP

Plasmid

#72945

Purpose

Level 3 Destination vector - Assembly of 2 Level 2 modules - AB

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 3 - A site

Use

Synthetic Biology

Tags

Expression

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTU3-B-RFP

Plasmid

#72946

Purpose

Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 3 - B site

Use

Synthetic Biology

Tags

Expression

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE146

Plasmid

#79449

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE167

Plasmid

#79457

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE168

Plasmid

#79458

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem no-pA

Plasmid

#80824

Purpose

CMVmin-promoted GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

CMV-GFPCAAX nopA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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GST-GW1D10(aa566-1343)

Plasmid

#21516

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contain aa566-1343

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
GST-TNR(aa1-204)

Plasmid

#21514

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contain aa1-204

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
GFP-GW1D1b(aa502-751)

Plasmid

#21521

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contain aa502-751

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMD19T-slr0230-PL31-sgRNANT1-KmR

Plasmid

#73221

Purpose

Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

PL31-sgRNA NT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL31

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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LI B-C N-NES KOZ b c7

Plasmid

#54445

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear Export Signal (NES) of heat stable inhibitor (PKI)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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GST-GW1D12(aa895-1211)

Plasmid

#21528

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contain aa895-1211

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
GST-GW1D1(aa254-751)

Plasmid

#21523

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contain aa254-751

Promoter

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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piHGpd/T101I

Plasmid

#1112

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 101 changed to isoleucine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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