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Plasmid#195158Purposemammalian expression of ER localized ABHD16A tagged with mNeonGreenDepositorInsertStim1NTD (STIM1 Human)
UseTagsABHD16A cytoplasmic domain and mNeonGreenExpressionMammalianMutationNonePromoterCMVAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p8271 LentiCRISPR v2 hygro sgSIGIRR-4
Plasmid#193980PurposeExpression of spCas9 and sgRNA targeting SIGIRRDepositorInsertspCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD411
Plasmid#178196PurposesRok overexpressionDepositorInsertsrok
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD231
Plasmid#178195PurposeRok overexpressionDepositorInsertrok
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK411.RR1
Plasmid#192644PurposeExpresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpRDepositorInsertBBa_J23119-RR1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB439
Plasmid#185093PurposeNMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::NMA111-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB280
Plasmid#185073PurposeLarger fusion of Nma111 nuclear localization signal to GFPDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111 aa 1-83PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB282
Plasmid#185074PurposeSmaller fusion of Nma111 nuclear localization signal to GFPDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111 aa 1-35PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB465
Plasmid#185098PurposeNMA111-GFP with NLS1 deleted under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111DNLS1-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB460
Plasmid#185094PurposeNMA111-GFP wild type under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB462
Plasmid#185095PurposeNMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::nma111Dnls1Dnls2-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB466
Plasmid#185099PurposeNMA111-GFP with NLS1 and NLS2 deleted under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111DNLS1DNLS2-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB467
Plasmid#185100PurposeNMA111-GFP with NLS2 deleted under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111DNLS2-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBT-Mb(GDx-Clo_L10)
Plasmid#183406PurposeExpresses Monobody GDx-Clo_L10 in E. coliDepositorInsertHis tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
UseTagsHis tag-Avi tag-TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 (BTLA ITIM) fused to mGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2711
Plasmid#175083PurposeMammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutationsDepositorInsertKSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationE223A, S226A, RC mutationsPromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2712
Plasmid#175084PurposeMammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutationsDepositorInsertKSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationE223A, S226A, RN2 mutationsPromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15484_HF-ABE8e
Plasmid#174122PurposeExpresses ABE8e fused with SpCas9-HF1DepositorInsertHF-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNArodA
Plasmid#149658Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only