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Showing: 4621 - 4640 of 16805 results

  1. pJBL7048

    Plasmid
    #167218
    Purpose
    cell-free nitrate sensing reporter
    Depositor
    Julius Lucks
    Insert
    pNar-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ydfJ115 promoter
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167218-plasmid-map-sequence-id-331012

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  2. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Gloria Rudenko
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressi…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    59733-plasmid-map-sequence-id-93744

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  13. pJBL7041

    Plasmid
    #167212
    Purpose
    overexpression of NarL in E. coli for cell-free nitrate biosensing
    Depositor
    Julius Lucks
    Insert
    NarL/FixJ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ydfl RR-DNA binding motif
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167212-plasmid-map-sequence-id-330997

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  16. pKPY738

    Plasmid
    #72674
    Purpose
    C. elegans let-858 3' UTR (Gateway Entry Clone)
    Depositor
    David Tirrell
    Insert
    let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72674-plasmid-map-sequence-id-133995

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  20. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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Showing: 4621 - 4640 of 16805 results