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Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEW3: pET-32a Dot1L(2-416)
Plasmid#124098PurposeDot1L(2-416) expression plasmidDepositorInsertDot1L(2-416) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL725
Plasmid#140395PurposeExpresses SmtB protein under the control of T7-lacO promoterDepositorInsertT7-lacO-SmtB-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
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bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBPCas2/3
Plasmid#89238PurposePlasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusionDepositorInsertCas2/3
UseTagsStrep II-MBPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL717
Plasmid#140387PurposeExpresses MobR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MobR-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-LIC-CHIKV-nsP2h
Plasmid#127935PurposeExpresses CHIKV nsP2h (1-465 aa) in bacterial cellsDepositorInsertCHIKV nsP2h
UseTagsHis6-Sumo-TEVExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEW4: pET-32a Dot1L(2-386)
Plasmid#124099PurposeDot1L(2-386) expression plasmidDepositorInsertDot1L(2-386) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1 Aquamarine
Plasmid#106285PurposeExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoterDepositorInsertAquamarine
UseTags6xHis and TEV cleavage (N terminal on insert)ExpressionBacterialMutationT65S and H148GPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
human MTAP
Plasmid#64077PurposeExpression of human 5'-methylthioadenosine phosphorylaseDepositorInsert5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
UseTagsHistidine tag and TEVExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1
Plasmid#87737PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046810
Plasmid#149251PurposeBacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-CRYM
Plasmid#31293DepositorInsertcrystallin, mu (CRYM Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only