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pcDNA3.1(+)-RasAR-NES
Plasmid#205567PurposeGenetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.DepositorInsertRasAR-NES (RAF1 Human, Synthetic)
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-GFP-CIBN
Plasmid#102252PurposeExpresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1
Plasmid#60540PurposeDonor vector for human MLK1DepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1 E179K
Plasmid#60541PurposeDonor vector for human MLK1 E179K mutantDepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationE179KPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab35 S22N inactive
Plasmid#47426DepositorInsertRab35 S22N (RAB35 Human)
UseTagsGFPExpressionMammalianMutationS22N inactivePromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1 D294A
Plasmid#60542PurposeDonor vector for human MLK1 D294A mutantDepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationD294APromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hATG7wt
Plasmid#87867PurposeExpress a wild type human Atg7/Apg7 in mammalian cellsDepositorInsertATG7 (ATG7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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PEX3*-SBP-GFP
Plasmid#120174PurposeExpresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)DepositorInsertPeroxisomal biogenesis factor 3 (PEX3 Human)
UseTagsSBP and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX
Plasmid#169729PurposeBacterial expression of N-terminally 6His tagged cDAXXDepositorInsertDAXX (DAXX Human)
UseTags6 HisExpressionBacterialMutationPromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP wildtype
Plasmid#73048PurposeC-terminal fusion of gfp Mouse wildtype Foxg1DepositorInsertmFoxg1 (Foxg1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT_KCNQ3
Plasmid#173163PurposeTo generate Flp-In CHO-KCNQ3 stable cellsDepositorInsertKCNQ3 (KCNQ3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX17FNV
Plasmid#206384PurposeExpresses human SOX17FNV in mammalian cells, for lentivirus generation.DepositorInsertSOX17FNV (SOX17 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p323.hSyn.Halo-mFMRP.Wpre
Plasmid#185909PurposeExpresses FMRP fused to HaloTag for protein localization through fluorescence imagingDepositorInsertHaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterhSyn (human Synapsin promoter)Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-HNRNPR
Plasmid#38067DepositorInsertHNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)
UseTagsFLAG, HA, and HisExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP_Rab35 Q67L active
Plasmid#47425DepositorInsertRab35 Q67L (RAB35 Human)
UseTagsGFPExpressionMammalianMutationQ67L activePromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC_P2A_Hygro_Barcode
Plasmid#120461PurposeBarcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMX-PL1-WDR5
Plasmid#59970PurposeMammalian Expression of WDR5DepositorInsertWDR5 (WDR5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only
