Addgene

We narrowed to 6,565 results for: ski

Showing: 4601 - 4620 of 6565 results

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pcDNA3.1/NDUFS7/myc-His

Plasmid

#127488

Purpose

Expresses myc-tagged NDUFS7 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS7 (NDUFS7 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA-CXCR4(90)

Plasmid

#98962

Purpose

Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Encodes a gRNA for CRISPR-mediated targeting of C…

Promoter

U6

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT2 CD

Plasmid

#106302

Purpose

Expresses SHMT2 K280A catalytic site mutant

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K280A catalytic site mutation, Silent mutations d…

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-NLS-VP16

Plasmid

#103821

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-VP16 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F1 3'UTR

Plasmid

#29468

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F1 3'UTR (E2F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK1 C121S

Plasmid

#53165

Purpose

Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C121S

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T3-HA-ERK2 GOF

Plasmid

#53200

Purpose

Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S/D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pLJM1 Flag GFP 4E-BP1 Rheb15

Plasmid

#112761

Purpose

express GFP-4EBP1-Rheb15

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)

Use

Lentiviral

Tags

Flag eGFP and Rheb15

Expression

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pXPR_BRD111

Plasmid

#78166

Purpose

Expresses Cas9 with the pLX311 backbone

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Cas9

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mutation

Promoter

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pPN007

Plasmid

#91575

Purpose

Express sgRNA targeting human BCL11A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BCL11A (BCL11A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMS04 [myo-3p::bPGC::SL2::mCherry]

Plasmid

#168172

Purpose

Expression of bPGC in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

bPGC

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181A

Plasmid

#105307

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S181A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CCR5-VN

Plasmid

#98963

Purpose

FLAG-tagged human CCR5 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

FLAG (dykdddd) epitope tag, Signal/leader sequenc…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pIG-707pUC19-tNGFR-P2A-TTC37

Plasmid

#186122

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

TTC37 (SKIC3 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-CHEK1 3'UTR

Plasmid

#29478

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CHEK1 3'UTR (CHEK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pNAS1b split mCherry NEDD4 WW2 pos ctrl

Plasmid

#112029

Purpose

Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Insert

NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry

Use

Tags

Split mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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