Showing: 4601 - 4620 of 4801 results
-
Plasmid#196053PurposeEncodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorInsertsUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
-
TRUPATH Triple Gagust
Plasmid#196054PurposeEncodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
anti-GFP scFv [N86/38] with sortase tag
Plasmid#204419PurposeMammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.DepositorInsertRecombinant mouse scFv targeting GFP (Aequorea victoria)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
anti-GFAP R416WT scFv [N206B/9] with sortase tag
Plasmid#204420PurposeMammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.DepositorInsertRecombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD Delta
Plasmid#214723PurposeExpresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertReceptor-Binding Domain (S SARS-CoV-2)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Quartet-SpyTag
Plasmid#214726PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag fusionDepositorInsertQuartet-SpyTag (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet
Plasmid#214727PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Alternate Quartet
Plasmid#214728PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Alternate Quartet
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet [SARS1]
Plasmid#214729PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Quartet [SARS1] (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Kraken Quartet
Plasmid#214730PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTagDepositorInsertSpyTag-Kraken Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet_NoLinker
Plasmid#214731PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTagDepositorInsertSpyTag-Quartet_NoLinker (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (C1204R)
Plasmid#61361Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; C1204R mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA TMEM 138-flag
Plasmid#41628DepositorInsertTransmembrane Protein 138 (TMEM138 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TMEM138-mCherry
Plasmid#41630DepositorInsertTransmembrane Protein 138 (TMEM138 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TMEM216-mCherry
Plasmid#41633DepositorInsertTransmembrane Protein 216 (TMEM216 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
Plasmid#103871PurposedPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4119-eGFP
Plasmid#108394PurposeGenomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293DepositorInsertPKD1 (PKD1 Mouse)
UseTagseGFPExpressionMammalianMutationvariant ID rs13468401 missense variant R4237QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 4601 - 4620 of 4801 results