Addgene

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pIOM14

Plasmid

#20868

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aacC1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pSL1394

Plasmid

#129522

Purpose

pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE3

Plasmid

#79442

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE1

Plasmid

#79440

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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AlphaB

Plasmid

#124524

Purpose

pColE1_AlphaB

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaD

Plasmid

#124526

Purpose

pColE1_AlphaD

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaE

Plasmid

#124527

Purpose

pColE1_AlphaE

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

Plasmid

#87586

Purpose

PV7 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSLBPres

Plasmid

#82454

Purpose

Encodes synthesized SLBP that is resistant to SMARTpool siRNA

Depositor

Bharat Ramratnam

Article

Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

Insert

SLBP (SLBP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMMRSF1-16

Plasmid

#20177

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

src homology domain 3 (CRK Human)

Use

Tags

His and NpuDnaEC16

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

NLS fusion

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus cerberus short

Plasmid

#24681

Depositor

Edward De Robertis

Article

Piccolo et al Nature. 1999 Feb 25. 397(6721):707-10.

Insert

cerberus (cer1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Cerberus short

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
SP_gRNA_pUC19_N_FancF_Left

Plasmid

#79892

Purpose

Human gRNA expression vector targeting FANCF (site 1 left)

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

FancF_Site_1_Left

Use

CRISPR

Tags

None

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSL1103

Plasmid

#129526

Purpose

pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Expression

Mutation

Promoter

Available since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBP-TL9

Plasmid

#74098

Purpose

TL9 RBS (AAAAAG)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

TL9 RBS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-SJM911

Plasmid

#72973

Purpose

Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

SJM911 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pUC-DEST-hSOX2-EGFP-PuroTK

Plasmid

#141026

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-YFP-HA no-pA

Plasmid

#82590

Purpose

3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

YFP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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