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Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE142
Plasmid#118494Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TagGFP2
Plasmid#121426PurposeTAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertempty TagGFP2
UseLentiviralTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#155384PurposedCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE130
Plasmid#118484Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-DEST(f)
Plasmid#190226PurposeGateway destination vector, empty AAV vector backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105
Plasmid#68559PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03
Plasmid#67137PurposeGateway destination vector for cell-free expression with N-term 8xHis eGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPO3
Plasmid#209977PurposeContains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pAMBeta1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK364.306
Plasmid#192642PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRA104
Plasmid#209337PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-DTA-DEST-R3R4(R)
Plasmid#141011PurposeMutisite Gateway mediated vector constructionDepositorInsertDTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB042 (FtsZ-mEos2)
Plasmid#49764PurposeInducible expression of FtsZ-mEos2 in bacteriaDepositorInsertFtsZ-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ906
Plasmid#44472DepositorInsertMinimal Mos1, unc-119, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB227
Plasmid#68618PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#104780PurposepSC218UG-At Ubiquitin10:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only