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Showing: 4581 - 4600 of 4616 results

  1. pMY-CD90.2-Rluc_miR

    Plasmid
    #163333
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter.
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163333-plasmid-map-sequence-id-322028

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  2. pGAPTrap-Luc2-IRESMeo

    Plasmid
    #82509
    Purpose
    Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    Luc2-IRESMeo
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82509-plasmid-map-sequence-id-156578

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  3. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid
    #163359
    Purpose
    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    Ly-6G (Ly6g Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    163359-plasmid-map-sequence-id-322106

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  4. pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

    Plasmid
    #163340
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    Ly-6G (Ly6g Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163340-plasmid-map-sequence-id-322050

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  5. psiCHECK-VIM270-pSM

    Plasmid
    #215871
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215871-plasmid-map-sequence-id-431666

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  6. psiCHECK-VIM270-pCM

    Plasmid
    #215869
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215869-plasmid-map-sequence-id-431661

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  7. pLenti-EF1a-CD90.2-Rluc_miR

    Plasmid
    #163360
    Purpose
    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    163360-plasmid-map-sequence-id-322108

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  8. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid
    #178185
    Purpose
    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.
    Depositor
    Irmela Jeremias
    Article
    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:
    Inserts
    GLuc
    CreERT2
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    178185-plasmid-map-sequence-id-351314

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  9. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  10. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Use
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

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  13. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  14. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  15. pdCas9-M-4A6

    Plasmid
    #73434
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4A6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73434-plasmid-map-sequence-id-135334

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  16. pdCas9-M-C4

    Plasmid
    #73427
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73427-plasmid-map-sequence-id-135327

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  17. pMX-mPGK-CD90.2-Rluc_miR

    Plasmid
    #163332
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter.
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mPGK
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163332-plasmid-map-sequence-id-322026

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  18. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

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  19. pMY-CD90.2-P2A-BlastDYK-Rluc_miR

    Plasmid
    #163341
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163341-plasmid-map-sequence-id-322055

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  20. pMX-CAG-CD90.2-Rluc_miR

    Plasmid
    #163331
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the CAG promoter.
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163331-plasmid-map-sequence-id-322021

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Showing: 4581 - 4600 of 4616 results