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Plasmid#182334PurposeGenetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.DepositorInsertDrFLINC-AKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synp-F-H2B-GCaMP6f
Plasmid#102994PurposeExpresses histone fused GCaMP6f under the synapsin promoterDepositorInsertH2B-GCaMP6f
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin I promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
UseTags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCD5-PR8-TEV-sfGFP-twinStrep
Plasmid#157933PurposeExpresses influenza A virus trimeric hemagglutinin fused to a sfGFPDepositorInsertA/PuertoRico/8/34 hemagglutinin (HA Synthetic)
UseTagssfGFP & TwinStrepExpressionMammalianMutationE190DPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-DIO-erHRP(N175S mutant)
Plasmid#79906PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)DepositorInserterHRP(N175S mutant)
UseAAV and Cre/LoxTagsHA tagExpressionMammalianMutationN175S mutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BHLHE40
Plasmid#110154PurposeExpression of human BHLHE40 in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMSCVneo-DEST
Plasmid#119746PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-SUR-YR
Plasmid#61768PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).DepositorInsertsSur gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromoterMagnaporthe oryzae ILV promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BHLHE40(S243A/E248A)
Plasmid#110155PurposeExpression of human BHLHE40 (S243A/E248A) in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
UseTagsHAExpressionMammalianMutationS243A/E248APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-GFP-α-Tubulin
Plasmid#213900PurposeImaging of Tubulin using Photoactivatable GFPDepositorInsertPA-GFP-α-Tubulin
UseTagsPhotoactivatable GFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lamp1_mStayGold
Plasmid#221902PurposeExpresses mStayGold fused to Lamp1DepositorInsertLamp1_mStayGold
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
mito-mStayGold_pTwistCMVBetaGlobWPRENeo
Plasmid#221898PurposeExpresses mStayGold targeted to the mitochondriaDepositorInsertcox8-mStayGold
UseTagsCox8ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo_aTubulin-pTwistCMVBetaGlobWPRENeo
Plasmid#221901PurposeExpresses Halo7 fused to alpha tubulinDepositorInsertHalo7-aTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lck-Halo_pTwistCMVBetaGlobWPRENeo
Plasmid#221900PurposeExpresses Halo7 targeted to the plasma membraneDepositorInsertLck-Halo7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo
Plasmid#221899PurposeExpresses alpha tubulin fused to mStayGoldDepositorInsertmStayGold-aTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA Display Part 1-4 DO
Plasmid#206514PurposeCcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cellsDepositorTypeEmpty backboneUseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only