Addgene

We narrowed to 4,110 results for: Abo

Showing: 881 - 920 of 4110 results

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BB2_L_DE_syn_BbsI

Plasmid

#89920

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_FG_syn_BbsI

Plasmid

#89922

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_GH_syn_BbsI

Plasmid

#89923

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHL

Plasmid

#64171

Purpose

harbours marker genes HIS3 and LEU2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDS3-X4

Plasmid

#70140

Purpose

Yeast chr. integration vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS3

Use

Tags

Expression

Yeast

Mutation

Promoter

AgTEF1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-GluA1-scFV-Halo

Plasmid

#230065

Purpose

Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

Depositor

Matthew Kennedy

Insert

Anti-GluA1 single chain variable fragment (scFv)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pPath-BGC08-SGE

Plasmid

#200006

Purpose

BGC08 pathway. SGE

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pITC

Plasmid

#192856

Purpose

Cloning and expression of transcription factor gene under control of naringenin inducible promoter

Depositor

Eveline Peeters

Article

Bernauw et al Methods Mol Biol. 2022;2516:113-141. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PfdeAR

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDF850

Plasmid

#228713

Purpose

Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

epPsaCas12f

Use

CRISPR

Tags

Expression

Mammalian

Mutation

I178A/K333V/K454P

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-GCaMP6f

Plasmid

#127874

Purpose

Fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET8

Plasmid

#107439

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET8 (MET8 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJKR-H-araC

Plasmid

#62563

Purpose

Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-SGE

Plasmid

#200003

Purpose

Violacein pathway. SGE

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTrcAgBIS (CO)

Plasmid

#35153

Depositor

Taek Soon Lee, Jay Keasling

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pHAHA

Plasmid

#12517

Depositor

Tong-Chuan He

Article

Luu et al Cancer Lett. 2005 Nov 8. 229(1):135-48.

Type

Empty backbone

Use

Tags

Double HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPPAT-mCherry

Plasmid

#167162

Purpose

Expresses human PPAT-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Pedley et al Methods Mol Biol. 2018;1764:279-289. doi

Insert

PPAT (PPAT Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-H-ttgR

Plasmid

#62565

Purpose

Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLM

Plasmid

#64168

Purpose

harbours marker genes HIS3, LEU2 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPath-pR-integrase

Plasmid

#200000

Purpose

Entry vector for biosynthetic pathway. pR-phiC31 integrase

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMLM802

Plasmid

#27203

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM800

Plasmid

#27202

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLUK

Plasmid

#64181

Purpose

harbours marker genes HIS3, LEU2, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-L-cdaR

Plasmid

#62558

Purpose

Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-C1-mCherry-LOVPLD

Plasmid

#219559

Purpose

ER-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

C1-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Use

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomMet14

Plasmid

#107451

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met14 (met14 Fission Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT11F

Plasmid

#165062

Purpose

Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE myc-Raptor S722A/S792A

Plasmid

#18117

Depositor

Reuben Shaw

Article

Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

Insert

Raptor (RPTOR Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S722A, S792A

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-cerMET3

Plasmid

#107436

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET3 (MET3 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-cerMET14

Plasmid

#107441

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET14 (MET14 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-cerMET2

Plasmid

#107435

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET2 (MET2 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 881 - 920 of 4110 results

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