Skip to main content
Addgene
Showing: 4561 - 4580 of 4616 results

  4. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid
    #222876
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FRB chain: V84R in GPA
    Promoter
    MSCV LTR
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222876-plasmid-map-sequence-id-441621

    Enlarge

  7. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid
    #165977
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165977-plasmid-map-sequence-id-328856

    Enlarge

  8. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155339-plasmid-map-sequence-id-360426

    Enlarge

  9. psiCHECK-VIM270-gSM

    Plasmid
    #215870
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215870-plasmid-map-sequence-id-431352

    Enlarge

  10. psiCHECK-VIM270-gCM

    Plasmid
    #215868
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215868-plasmid-map-sequence-id-431664

    Enlarge

  16. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid
    #165976
    Purpose
    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pphlO6), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165976-plasmid-map-sequence-id-328850

    Enlarge

  17. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #105000
    Purpose
    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105000-plasmid-map-sequence-id-203163

    Enlarge

Showing: 4561 - 4580 of 4616 results