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We narrowed to 30,578 results for: CMV

Showing: 4561 - 4580 of 30578 results

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pCDH1-CMV-FIP200-V44D-mCherry-Hygro

Plasmid

#210853

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

V44D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

Plasmid

#208974

Purpose

Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

B103-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

Plasmid

#208973

Purpose

Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

Plasmid

#208971

Purpose

Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Phi29(-exo;D169A)

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ115-133, 204-217 PPM1H

Plasmid

#207343

Purpose

Mammalian expression of HA-Δ115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Δ115-133, Δ204-217

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

Plasmid

#205442

Purpose

production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

Depositor

Jeremy Kay

Article

Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

Insert

Unc5c (Unc5c Mouse)

Use

AAV

Tags

3xHA

Expression

Mutation

Promoter

mini-CMV (with MVM small intron)

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

Plasmid

#205417

Purpose

Mutagenesis of Ntsr1, second guide

Depositor

Larry Zweifel

Insert

Ntsr1 (Ntsr1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Flag-LRRK2 K17A/K18A/R1441G

Plasmid

#186013

Purpose

Flag-LRRK2 K17A/K18A/R1441G

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)

Use

Tags

Expression

Mammalian

Mutation

See depositor's comments below.

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCA31-pCS2-CMV-stdPCP-p65-HSF1

Plasmid

#199462

Purpose

Expression of stdPCP-PH in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA30-pCS2-CMV-stdMCP-p65-HSF1

Plasmid

#199461

Purpose

Expression of stdMCP-PH in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdMCP-PH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

Plasmid

#199460

Purpose

Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

Zebrafish NarTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

Plasmid

#192133

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-KY

Use

AAV

Tags

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (K390A, Y446A)

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Tags

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CMV CCR6-eLOV-TEVcs-Gal4

Plasmid

#173113

Purpose

Expresses CCR6 receiver construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)

Plasmid

#191174

Purpose

Mammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

Plasmid

#191161

Purpose

Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP.t2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

Plasmid

#169589

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-2

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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