Addgene

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pT7-EGFP-C1-HsNot7-T142Y_R

Plasmid

#147381

Purpose

Mammalian Expression of HsNot7-T142Y

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-T142Y (CNOT7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1595-2376_K

Plasmid

#146808

Purpose

Mammalian Expression of HsNot1iso1_1595-2376

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1595-2376 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)_G

Plasmid

#146407

Purpose

Bacterial Expression of HsDCP1a_539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation T1716G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G

Plasmid

#146409

Purpose

Bacterial Expression of HsDCP1a_539-582-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut2 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation T1716G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G

Plasmid

#146411

Purpose

Bacterial Expression of HsDCP1a_539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582 (DCP1A Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation T1716G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1554

Plasmid

#143294

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SP110 (SP110 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0234

Plasmid

#142487

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MNX1 (MNX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-WT

Plasmid

#190094

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR Wild Type (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-S12AH19A_V

Plasmid

#147804

Purpose

Mammalian Expression of HsDCP1a-S12AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-S12AH19A (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-mDia1-GBD (1-305 aa) in pEGFPN1

Plasmid

#187280

Purpose

Express EGFP-mDia1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

mDia1 (Diaph1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GBD (1-305aa)

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190078

Purpose

express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Rhinolophus ferrumequinum)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V

Plasmid

#147805

Purpose

Mammalian Expression of HsDCP1a-S12AQ18AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-S12AQ18AH19A (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-M141R_R

Plasmid

#147379

Purpose

Mammalian Expression of HsNot7-M141R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-M141R (CNOT7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot7_9-260_R

Plasmid

#147369

Purpose

Bacterial Expression of HsNot7_9-260

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7_9-260 (CNOT7 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Clim Actin MCH Puro (CAMP)

Plasmid

#104446

Purpose

Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.

Depositor

Inaki Ruiz-Trillo, Elena Casacuberta

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

mCherry

Use

Expression in corallochytrium (single-celled euka…

Tags

Expression

Mutation

Promoter

actin

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MNX1-Adel-IDR

Plasmid

#145247

Purpose

Expresses fusion of GAL4 DNA-binding domain and MNX1-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MNX1-Adel-IDR (MNX1 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LIC-Z-delCry2

Plasmid

#153545

Purpose

this is the non-clustering version of LIC-Z tagged with mcherry

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

with Cry2 sequence deleted from LIC-Z

Promoter

CMV

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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563aa ELL2 pEF4B

Plasmid

#127273

Purpose

Expresses 563aa human WT ELL2

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 truncated at SphI (ELL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Contains 564 N-terminal amino acids ELL2, termina…

Promoter

pEF1a

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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