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Plasmid#182589Purposeexpresses bfp-hcoilin in mammalian cellsDepositorInsertCoilin (COIL Human)
UseLentiviralTagsBFPExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 KD, dominant negative chk2
Plasmid#41902DepositorInsertChk2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationD347APromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-GalT
Plasmid#87327PurposeTo express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferaseDepositorInsert1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRIM33-GFP
Plasmid#65399PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM33 (TRIM33 Human)
UseTagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM28
Plasmid#65397PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM28 (TRIM28 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-TIR-AAA
Plasmid#187901PurposeMammalian cell expression of a triple mutant of p62: P228A, E230A, P232ADepositorInsertSQSTM1 (SQSTM1 Human)
UseTagsmCherryExpressionMammalianMutationTriple alanine mutant: P228A, E230A, P232APromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R
Plasmid#63565PurposeExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysines at 5, 9, 118, 119, 125, 127, and 129 are…PromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1_ICD
Plasmid#121480Purposeexpression of human PD-L1 in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseTagsEGFPExpressionMammalianMutationTM+ICDPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1_ECD
Plasmid#121479Purposeexpression of human PD-L1 in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseTagsEGFPExpressionMammalianMutationECD+TMPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD2sE_SC10
Plasmid#219716PurposeDV2 stable E dimer with fusion loop mutationDepositorInsertDV2 sE SC10
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, T280PPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mGreenLantern
Plasmid#164468PurposeCre-dependent EF1a-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVTagsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterEF1aAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-WT-V5
Plasmid#167468Purposeectopic expression of hDDB2 in mammalian cellsDepositorInsertDDB2 (DDB2 Human)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATG-3925 (CBG2)
Plasmid#167839PurposeExpresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cellsDepositorInsertClick Beetle Green 2
UseTags6-HisExpressionBacterial and MammalianMutationQ348H. Click Beetle Green 2 (CBR2) has 3 amino a…PromoterCMV, T7Available sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hCdt1-HA
Plasmid#117500PurposeExpresses human Cdt1 fused to HA tagDepositorInsertCdc10-dependent transcript-1 (CDT1 Human, 1700 bp)
UseTagsHAExpressionMammalianMutationwild-typePromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
tetO-GATA6
Plasmid#170688Purposedoxycycline-inducible overexpression of GATA6DepositorInsertGATA6 (GATA6 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAG, mCherry, and pHluorinExpressionMammalianMutationPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit B3SE
Plasmid#49171PurposepHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
UseTagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationGTG (V) to TGC (C)PromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only