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Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBK.136
Plasmid#187220PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectivelyDepositorInsertRetron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseTagsExpressionBacterialMutationPromoterA: pSalTTC; B: pTet*Available sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW045
Plasmid#185690PurposeT-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAsDepositorInsertCas9, Drm1b gRNAs
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW037
Plasmid#185688PurposeT-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAsDepositorInsertCas9, Drm1b gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
UseTagsExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti NL
Plasmid#113450PurposeLentiviral NanoLuc control expression vectorDepositorInsertNanoLuc
UseLentiviral and LuciferaseTagscmycExpressionMammalianMutationPromoterhUbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG17) PAGER(TF)
Plasmid#229997PurposeExpresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG2) PAGER(TF)
Plasmid#229996PurposeExpresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Dual-iCas9-CMV
Plasmid#167500PurposeExpression of dual inducible Cas9 with CMV promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA
Plasmid#104097PurposeExpresses TREAT-PK co-localization control in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR and 24xPP7 stem l…ExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Dual-iCas9-EFS
Plasmid#167501PurposeExpression of dual inducible Cas9 with EFS promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianMutationPromoterEFSAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_Rab11a
Plasmid#65705PurposeExpresses YNL-Rab11a in mammalian cellsDepositorInsertRab11a (Rab11a Mouse)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Dual-iCas9-hALB
Plasmid#167502PurposeExpression of dual inducible Cas9 with hALB promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianMutationPromoterhALBAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc-NL-GW
Plasmid#113446PurposeNanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLuciferase; Gateway shuttle vectorTagscmyc-NanoLucExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-NL-myc
Plasmid#113447PurposeNanoLuc Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLuciferase; Gateway shuttle vectorTagsNanoLuc-cmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti myc-NL-GW
Plasmid#113454PurposeLentiviral NanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviral and LuciferaseTagscmyc-NanoLucExpressionMammalianMutationPromoterhUbCAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_non-targeting
Plasmid#140109PurposeCRISPR-Cas9 library validation (negative control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only