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Bacterial Strain#62078PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.DepositorBacterial ResistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only
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RF5
Bacterial Strain#62073PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB hisG genes deleted.DepositorBacterial ResistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE
Plasmid#202198PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotorDepositorInsertstChrimsonR, PdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE
Plasmid#202199PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombinationDepositorHas ServiceAAV1 and AAV5InsertstChrimsonR, PdCO
UseAAV and Cre/LoxTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-DHFR
Plasmid#20217DepositorInsertDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-3xDHFR
Plasmid#20219DepositorInsert3xDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-2xDHFR
Plasmid#20218DepositorInsert2xDHFR (folA E. coli)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQE30-VRN1-R249E-R289E-R296E
Plasmid#159532PurposeExpresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coliDepositorInsertVERNALIZATION1 (VRN1 Mustard Weed)
UseTagsSix-Histidine tagExpressionBacterialMutationThree charge reversal mutations, namely R249E R28…PromoterT5Available sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNoxa1
Plasmid#58531Purposeexpresses human Noxa1 in mammalian cellsDepositorInsertNADPH oxidase activator 1 (NOXA1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 FLIPglu-600u
Plasmid#13572DepositorInsertFLIPglu F16A
UseTagsECFP and EYFPExpressionMammalianMutationF16A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNoxa1
Plasmid#58339Purposeexpresses mouse Noxa1 in mammalian cellsDepositorInsertNADPH oxidase activator 1 (Noxa1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xDHFR-myc
Plasmid#20216DepositorInsert3xDHFR (folA E. coli)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
2xDHFR-myc
Plasmid#20215DepositorInsert2xDHFR (folA E. coli)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat- (E318Q)
Plasmid#41887DepositorInsertKai C CII cat (E318Q)
UseBl de3 21TagsExpressionBacterialMutationchanged amino acid 318 E to QPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAIDA1
Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
UseTagsHis and MycExpressionBacterialMutationPromoterlacUV5Available sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNoxo1
Plasmid#58530Purposeexpresses human Noxo1 in mammalian cellsDepositorInsertNADPH oxidase organizer 1 (NOXO1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_EcSSPB-nano_X
Plasmid#131212PurposeExpresses EcSSPB-nano (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only