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Plasmid#52051PurposeCMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusionDepositorInsertUBR5 ∆HECT (UBR5 Human)
UseTagsGFPExpressionMammalianMutationC2769APromoterCMVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPExpressionMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2
Plasmid#183352PurposeFlp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)DepositorInsertTVA, EGFP and tTA
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB3
Plasmid#120288PurposeProduce human recombinant TGFB3 in DE3 cellsDepositorInsertTransforming Growth Factor-β3 (TGFB3 Synthetic, Human)
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
MGAT2-RpHLuorin2
Plasmid#171718PurposeCis-/medial-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8m
Plasmid#169248PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.DepositorInsertRiboL1-jGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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piRFP670-N1-GalT
Plasmid#87325PurposeTo express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferaseDepositorInsert1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
UseTagsiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…PromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG104 FAST
Plasmid#130720PurposeExpresses FAST (also called YFAST) in mammalian cellsDepositorInsertFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-DDX54-FLAG/HA
Plasmid#97062PurposeExpresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-GCaMP7f-CCR5TC
Plasmid#216534PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsGCaMP7fExpressionMammalianMutationPromoterCAGAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
hRAD18 dC2-EGFP
Plasmid#68826PurposeExpresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFPDepositorInserthuman RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA2 (SMARCA2 Human)
UseTagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit g2SE
Plasmid#49170PurposepHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
UseTagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only