Showing: 4441 - 4460 of 7190 results
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pT3TS-zAsCpf1
Plasmid#107185Purposeplasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)DepositorInsertAsCpf1
UseCRISPRTagsNLS-3xHAExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSP64T-hLbCpf1
Plasmid#107187Purposeplasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)DepositorInserthLbCpf1
UseCRISPRTagsNLS-3xHAExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pPFK300_Cas9
Plasmid#104911PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT
Plasmid#87259PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133DepositorInsertsYFP-mAID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and mAIDExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailabilityAcademic Institutions and Nonprofits only -
Lenti-AsCpf1-Blast
Plasmid#84750PurposeExpresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertAsCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianMutationPromoterEFS-NSAvailabilityAcademic Institutions and Nonprofits only -
Lenti-LbCpf1-Blast
Plasmid#84751PurposeExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertLbCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianMutationPromoterEFS-NSAvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Cdt1
Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1ExpressionMutationcodon optimized for humanPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p28-2iDMD-puro
Plasmid#88895Purpose28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.DepositorInsertsDystrophin
luciferase
puromycin resistance enzyme
mCherry
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM_hIRF-1
Plasmid#92221PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoterDepositorInsertsSp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6AvailabilityAcademic Institutions and Nonprofits only -
p46Cpf1-OP2
Plasmid#98592PurposeProduces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.DepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationCodon optimized for E. coliPromoteraraBADAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-gp120(BaL)-CD4
Plasmid#98957PurposeMammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolateDepositorInsertHIV-1 (BaL) gp120
UseTagsCD4 (domains D1-D2), CD5 leader sequence, FLAG ep…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
UseTagsStrep(II)-tagExpressionBacterialMutationPromoterT7 PromotorAvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)
Plasmid#99144PurposeInducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139ADepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailabilityAcademic Institutions and Nonprofits only
Showing: 4441 - 4460 of 7190 results