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pSBTet(-ĪrtTA)-GB dTomato-P2A-CaMXMT
Plasmid#139176PurposeSB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ĪrtTA)-GB backbone.DepositorInsertCaMXMT
UseTransposonTagsdTomato-P2AExpressionMammalianMutationP131T on dTomato (Please see depositor comments)Promotertight TRE-controlled CMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC-LIC+
Plasmid#62312PurposeE. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage siteDepositorTypeEmpty backboneUseTags6xHis and thrombin protease cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Nucleus-7
Plasmid#54206PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsmEmerald-NucleusExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsEBFP2-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
U25m
Plasmid#121027PurposeMoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRiboJ + UTR1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJO-7
Plasmid#31992DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCer-C3-Plk1
Plasmid#68132PurposeExpression of Cerulean-tagged Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationwild-typePromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GST
Plasmid#62329PurposeE. coli pACYC based expression vector with N-terminal TEV cleavable GST-tagDepositorTypeEmpty backboneUseTagsGlutathione S-transferase (GST) tag and TEV proteā¦ExpressionBacterialMutationPromoterT7-lac0Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHL1726
Plasmid#53032PurposePLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and hfqleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcB
Plasmid#51282PurposeE. coli expression vector for E. coli RtcB RNA ligaseDepositorInsertE. coli RtcB
UseTags6xHisExpressionBacterialMutationPromoterT7 RNA PolymeraseAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF_6xHIS_Csx29
Plasmid#197613PurposeFor protein purification of Csx29DepositorInsert6-HIS tagged Csx29
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSirt6-GFP
Plasmid#20275DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only
