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Plasmid#85393PurposeMTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertRGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorUseTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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mCardinal-Cx26-7
Plasmid#56156PurposeLocalization: Gap Junctions, Excitation: 604, Emission: 659DepositorInsertCx26
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-CAF1-C-10
Plasmid#56153PurposeLocalization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659DepositorInsertCAF1
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-Actin-7
Plasmid#57570PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKO-Alpha-Actinin-19
Plasmid#57831DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
XE224 human flag-Chibby-pCS3+FLT
Plasmid#16824DepositorInsertflag-Chibby (CBY1 Human)
UseXenopus expressionTagsflagExpressionMutationPromoterAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s
Plasmid#40753PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6s
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6m
Plasmid#40754PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6m
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NIR-GECO2G
Plasmid#159605PurposeAAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2GDepositorInsertNIR-GECO2G
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mKeima-LGALS3
Plasmid#175780PurposeLentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic fluxDepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF-miRFP709-hCdt(1/100)
Plasmid#80007PurposehCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)DepositorInserthCdt1(1/100) (CDT1 Human)
UseLentiviralTagsmiRFP709ExpressionMutationPromoterEF-1 alphaAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-CFP;UBC-Cre25nt
Plasmid#65727PurposeExpresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.DepositorInsertsCMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression
UseCre/LoxTagsExpressionMammalianMutationPromoterCMV and UBCAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only