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Plasmid#59989PurposeSox1/2/3 (SoxB1) driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Sox1/2/3 (SoxB1) -2373/+3Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcB-His-CD)
Plasmid#176194Purposeexpresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
UseTagsGfcB-6xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-10xHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h2
Plasmid#86691PurposeFor transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
UseTagsHAExpressionMutationPromoterT3Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA
Plasmid#121954PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH2644
Plasmid#179632PurposePnmr-1::TetanusToxin-UrSL-wCherry unc-54 3' UTR C.elegans AVA/E/D premoter IN and others expression of TetanusToxin-UrSL-wCherryDepositorInsertTetanusToxin
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAN101
Plasmid#131123PurposePlasmid for overexpression of prion domains to induce prionsDepositorTypeEmpty backboneUseTagsmKate2ExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgMUC4_dual-MTS-3x-Set I
Plasmid#162762PurposeSeparately expressing 3 unique sgMUC4_dual-MTS (SetI)DepositorInsertsgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABEmax_IVT
Plasmid#171761PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymeraseDepositorInsertABEmax
UseVector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miRNT
Plasmid#177808PurposeA nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-NT
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
WFS1 P724L-Flag-pcDNA3
Plasmid#13012DepositorInsertWolfram Syndrome 1 (WFS1 Human)
UseTagsFlagExpressionMammalianMutationchanged P 724 to LPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEasy Flox
Plasmid#11725DepositorInsertpEasyFlox
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-end
Plasmid#71512PurposeMammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.DepositorInsertConnexin 35
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT15485_Hypa-ABE8e
Plasmid#174123PurposeExpresses ABE8e fused with Hypa-SpCas9DepositorInsertHypa-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTP130 (pTA106_TnsABC(Tn6900))
Plasmid#164261PurposeVector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.DepositorInserttnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutation(tnsA) Changed Alanine 125 to Aspartic acidPromoterLactoseAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGEM-sgMUC1_dual-MTS-3x-Set I
Plasmid#162763PurposeSeparately expressing 3 unique sgMUC1_dual-MTS (SetI)DepositorInsertsgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_mito
Plasmid#113930PurposePlasmid encoding the mictochonfrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cellsDepositorInsertH-Luc_mito
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only