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Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hu-c-Myc-dN1
Plasmid#50773Purposeretroviral expression of human c-Myc dN1 fragmentDepositorInsertc-Myc -dN1 (MYC Human)
UseRetroviralTagsExpressionMammalianMutationN-terminal deletion, contains aa 14-439PromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S249
Plasmid#58908Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S249 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSN69
Plasmid#100125Purposeexpresses putative cMYC DBD with a HA tagDepositorInsertmCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
UseLentiviralTagsExpressionMammalianMutationInsert corresponds to the DBD of cMYCPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-NEMO-C54/347A
Plasmid#27268DepositorInsertNEMO (IKBKG Human)
UseTagsFLAGExpressionMammalianMutationCys54Ala Cys347AlaPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TMED1-bio-His
Plasmid#51832PurposeExpresses full-length Transmembrane emp24 domain-containing protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED1 (TMED1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(2Q)
Plasmid#21752DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains 2Q (2 glutamines) and there isno …PromoterCMVAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-Fibrillarin-C1
Plasmid#134538PurposeExpresses YFP-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756K
Plasmid#60727PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-DAXX-D/E-His
Plasmid#179619PurposeExpress DAXX D/E region in bacteriaDepositorInsertdeath domain associated protein (DAXX Human)
UseTagsGST, His, and TEVExpressionBacterialMutationamino acids 414-505 of DAXXPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLINK CDK9 myc (D167N) (P#1084)
Plasmid#14637DepositorInsertcdk9 D167N (CDK9 Human)
UseTagsmycExpressionMammalianMutationD167N. Kinase negative mutant.PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
RB-409-815-pGex2T
Plasmid#21784DepositorInsertRb (RB1 Human)
UseTagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 409-815 of wt Rb …PromoterAvailable sinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Farnesyl-5
Plasmid#57613PurposeLocalization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertFarnesyl (HRAS Human)
UseTagstdEosExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV HA hRb delta CDK + S230
Plasmid#58907Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S230 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T252
Plasmid#58910Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with T252 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
RIPK3 gRNA (BRDN0001148666)
Plasmid#76999Purpose3rd generation lentiviral gRNA plasmid targeting human RIPK3DepositorInsertRIPK3 (RIPK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF myc Gal CDK9 (P#1475)
Plasmid#14631DepositorInsertcdk9 (CDK9 Human)
UseTagsGal and mycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only