-
Plasmid#195133PurposegRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherryDepositorInsertMDM4 CRISPRi gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_CRISPRi_gRNA_1
Plasmid#195132PurposegRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherryDepositorInsertMDM4 CRISPRi gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Downstream_gRNA_1
Plasmid#195136PurposegRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Downstream gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Upstream_gRNA_2
Plasmid#195135PurposegRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Upstream gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Upstream_gRNA_1
Plasmid#195134PurposegRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Upstream gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE-N1-RDH11
Plasmid#161916PurposeExpresses untagged human RDH11. Confers G418 resistance.DepositorInsertRetinol dehydrogenase 11 (RDH11 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-RDH11
Plasmid#161924PurposeTo generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.DepositorInsertsgRNA targeting RDH11 exon 2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v4
Plasmid#173788PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 4DepositorInsertSpike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ287-RVR
Plasmid#138124PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoterDepositorInsertBsCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN512R, K518V and N522RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ285-RVR
Plasmid#138121PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoterDepositorInsertLb5Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN512R, K518V and N522RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ233-STU
Plasmid#138111PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminatorDepositorInsertdLbCas12a-SRDX
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-STU
Plasmid#138112PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminatorDepositorInsertFnCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP
Plasmid#91884PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1DepositorInsertgRNA targeting Gfi1 (Gfi1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP
Plasmid#91877PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1DepositorInsertgRNA targeting Gfi1 (Gfi1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP
Plasmid#91883PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1DepositorInsertgRNA targeting Gfi1 (Gfi1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPANXN4_WT
Plasmid#81998PurposeGateway Donor vector containing SPANXN4, part of the Target Accelerator Plasmid Collection.DepositorInsertSPANXN4 (SPANXN4 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PSG2_WT
Plasmid#81917PurposeGateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.DepositorInsertPSG2 (PSG2 Human)
UseGateway entry vectorTagsExpressionMutationV20L; p.333_335del-NPT-ins-IQHPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FAM200A_WT
Plasmid#81878PurposeGateway Donor vector containing FAM200A ,part of the Target Accelerator Plasmid Collection.DepositorInsertFAM200A (FAM200A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SMR3A_WT
Plasmid#81887PurposeGateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.DepositorInsertSMR3A (SMR3A Human)
UseGateway entry vectorTagsExpressionMutationC42R; P132LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPATA8_WT
Plasmid#81866PurposeGateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.DepositorInsertSPATA8 (SPATA8 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only