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Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-gRNA-EF1a-EGFP-CAAX
Plasmid#167936PurposegRNA entry vectorDepositorInsertU6-gRNA backbone
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-EEA1 wt
Plasmid#42307DepositorInsertEEA1 (EEA1 Human)
UseTagsGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_COCVmut_G
Plasmid#207262PurposeMammalian expression of a mutant Cocal virus G proteinDepositorInsertCOCVmut_G
UseTagsExpressionMammalianMutationK47A/Y209A/R354A; mammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Control Vector
Plasmid#212937PurposeControl vector with constitutive firefly luciferase expressionDepositorInsertluc+
UseLuciferaseTagsExpressionMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)
Viral Prep#51085-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
HLCS_pCSdest
Plasmid#53803DepositorInsertHLCS (HLCS Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV5)
Viral Prep#125712-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromotershort version of αCamKinase II promoter (0.4kb)TagsmScarletAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe10_ChR2_mCherry
Plasmid#213815PurposeAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherryDepositorInsertChannelrhodopsin 2-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV9)
Viral Prep#114471-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_C1
Plasmid#85044PurposeMammalian expression of mScarlet-iDepositorHas ServiceCloning Grade DNAInsertmScarlet-i
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1
Plasmid#159130Purposeexpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2069
Plasmid#141902PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCuo CA Rac1
Plasmid#84643Purposeconstitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.DepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationConstitutively Active, Q61LPromotercumate-inducible promoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV2)
Viral Prep#107738-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only