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Plasmid#221013Purposemammalian expression vector of myc-Flag tagged MMS22L siRNA resistantDepositorInsertMMS22L (MMS22L Human)
UseTagsFlag and mycExpressionMammalianMutationsilent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…PromoterCMVAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CIRTS-control
Plasmid#213788PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-Gas5
Plasmid#213172PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-Gas5 gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiBIRC6-1
Plasmid#202447PurposeExpression of a CRISPRi guide targeting BIRC6DepositorInsertBIRC6 gRNA (BIRC6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2(3)-C911
Plasmid#202452Purposea seed-matched control for pRSITEP-puro-shBIRC6-2(3)DepositorInsertBIRC6 shRNA (BIRC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2(3)
Plasmid#202451PurposeDOX-inducible expression of a short-hairpin RNA targetinging human BIRC6DepositorInsertBIRC6 shRNA (BIRC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgBIRC6-1
Plasmid#202437PurposeExpression of a guide RNA targeting BIRC6DepositorInsertBIRC6 gRNA (BIRC6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_016-sgHRI
Plasmid#202443PurposeExpression of a guide RNA targeting HRIDepositorInsertHRI gRNA (EIF2AK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2
Plasmid#202449PurposeDOX-inducible expression of a short-hairpin RNA targetinging human BIRC6DepositorInsertBIRC6 shRNA (BIRC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2-C911
Plasmid#202450Purposea seed-matched control for pRSITEP-puro-shBIRC6-2DepositorInsertBIRC6 shRNA (BIRC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiBIRC6-5
Plasmid#202448PurposeExpression of a CRISPRi guide targeting BIRC6DepositorInsertBIRC6 gRNA (BIRC6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgBIRC6-4
Plasmid#202438PurposeExpression of a guide RNA targeting BIRC6DepositorInsertBIRC6 gRNA (BIRC6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgBIRC6-5
Plasmid#202439PurposeExpression of a guide RNA targeting BIRC6DepositorInsertBIRC6 gRNA (BIRC6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgKCMF1
Plasmid#202441PurposeExpression of a guide RNA targeting KCMF1DepositorInsertKCMF1 gRNA (KCMF1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2.2
Plasmid#189842PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2.3
Plasmid#189843PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP8.3
Plasmid#189848PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-S212D
Plasmid#197515PurposeMammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)DepositorInsertBCL2 interacting protein 3 like (BNIP3L Human)
UseTagsMycExpressionMammalianMutationSerine 212 changed to aspartic acidPromoterCMVAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-S212A
Plasmid#197514PurposeMammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)DepositorInsertBCL2 interacting protein 3 like (BNIP3L Human)
UseTagsMycExpressionMammalianMutationSerine 212 changed to alaninePromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Downstream_gRNA_2
Plasmid#195137PurposegRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Downstream gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only